Metabolic  Engineering of Industrial Yeast Strains

“Industry is awaiting the microorganism that can produce high levels of ethanol from both pentose and hexose sugars released lignocellulosic.” We have engineered yeasts to metabolise both pentose and hexose sugars released lignocellulosic waste for the production of bioethanol. We can tailor such strains to add value to either waste or co-products.

cellulaseAlcoholProduction

Fermentation of phosphoric acid swollen cellulose (PASC) by Cel 2.0 strains. Recombinant yeast strain CM51 expressing different Cel 2.0 gene cassettes were co-cultured or separately for 48 h. Pooled medium from Cel 2.0 cells (grey columns) or the Cel 2.0 co-culture medium (black columns) were added to PASC (1000 g l1 PASC), incubated at 50°C for 24 h and then fermented at 30°C by addition of fresh CM51 cells expressing the Cel 2.0 gene cassettes. The clear columns represent control strains that do not express Cel 2.0 cassettes. Similarly, we also have ethanol producing recombinant lager yeast strains that metabolize the xylose sugars from the hemicellulose fraction of cellulosic waste. For details, refer to link.